On the following days of 2025 are closed venues of the Prague Jewish Museum and Jewish community are closed:
| April 13th | 1st day of Passover |  |
| April 14th | 2nd day of Passover | |
| April 19th | 7th day of Passover | |
| April 20th | 8th day of Passover | |
| June 2nd | 1st day of Shavuot | |
| June 3rd | 2nd day of Shavuot | |
| September 23rd | 1st day of Rosh Hashanah | |
| September 24th | 2nd day of Rosh Hashanah | |
| October 2nd | Yom Kipur | |
| October 7th | 1st day of Sukkot | |
| October 8th | 2nd day of Sukkot | |
| October 14th | Shemini Atzeret | |
| October 15th | Simchat Torah |
